Retrograde Tracing of <em>Drosophila</em> Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
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چکیده
منابع مشابه
Characterization of serotonergic neurons using concurrent fluorescent retrograde axonal tracing and immunohistochemistry.
The recent introduction ofa number offluorescent retrograde axonal tracers that have different emission spectra (1,2,9,10,25,26) has permitted the investigation of possible collateral projections of senotonengic neurons. Extensive collateralization from single neurons in the raphe nuclei was expected given the widespread distribution ofserotonergic nerve terminals in the central nervous system ...
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Fluorescent lipophilic dyes, such as DiI, stain cellular membranes and are used extensively for retrograde/anterograde labeling of neurons as well as for marking the position of extracellular electrodes after electrophysiology. Convenient histological clearing techniques, such as CLARITY, enable immunostaining and imaging of large volumes for 3D-reconstruction. However, such clearing works by r...
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Copyright © 1995 by J. Coppeta. Published by the American Institute of Aeronautics and Astronautics, Inc. with permission. Abstract In this paper a method of quantifying fluid mixing using the fluorescence intensity of fluorophors in aqueous solution is presented. The general technique involves ratioing optically separable fluorescence from two fluorescent dyes. The resulting ratio normalizes ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2020
ISSN: 1940-087X
DOI: 10.3791/60716